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Image sequence of cell recognition and tracking. (A) A cell is selected by the operator. (B) Cell is recognized using Hough gradient transform (highlighted red). A small square is used to denote the region of interest for image processing. (C) Cell is visually servoed to the image center. White dots are cell trajectories. (D) Cell reaches the center of the image. (E–F) partial cell aspiration and (G) deposition into a microwell.
