Table 2.

Nucleotide sequence of the primers used in the MSP experiments.

Type of primer	Nucleotide sequence (5-3)
DSE-UF	GTGGAGGTGATGTTGGAGAGAAT
DSE-MF	AGGCGACGTCGGAGAGAAC
DSE-UR	AAACTACCCACCCAAAACTCCCA
DSE-MR	TACCCGCCCGAAACTCCCG
D4ST-UF	GGGTGGAGAGTGGTTGGGT
D4ST-MF	GTGGAGAGCGGTCGGGC
D4ST-UR	AATAACCAACTCCCAAACTACAACA
D4ST-MR	CCGACTCCCGAACTACGACG
C4ST-UF	TTAGATGGTGGTTGAGTTT
C4ST-MF	TTAGACGGCGGTCGAGTTC
C4ST-UR	AAAATATAATCACATCCAACTATTAACCA
C4ST-MR	ATCGCGTCCAACTATTAACCG

The longer segments of the unmethylated primers are in boldface type and differences between methylated/modified and ummethylated/modified sequence are underlined underlined