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FRET between CPM-MtTOP1-K524C and fluorescein labeled oligonucleotides. Increase in fluorescein fluorescence (520 nm) was observed when CPM-MtTOP1-K524C was titrated with increasing concentrations of oligo internally labeled with fluorescein indicating the occurrence of FRET (A). Little or no increase in fluorescein fluorescence was observed when titrations were carried out with oligo labeled at the 3' end (B) or the 5' end (C).
