Table 2.
Proportions of single Aβ peptide species secreted by non-adherent mononuclear phagocytes stimulated with polystyrene beads, acLDL or LPS (2D-Aβ-WIB, mab 1E8)
| CON (n = 5) |
Polystyrene beads (n = 5) |
acLDL (n = 4) |
LPS (n = 4) |
Polystyrene beads + LPS (n = 4) |
|||
|---|---|---|---|---|---|---|---|
| Spot | pI | Aβ identity proposed | % of total Aβ | ||||
| 5.37-A | 5.37 | Aβ1-40 | 63.9 ± 3.8 | 30.0 ± 7.0*** | 37.5 ± 6.5*** | 49.2 ± 9.5* | 42.0 ± 12.0** |
| 5.37-B | 5.37 | Aβ1-42 | 3.7 ± 1.9 | 2.8 ± 0.8 | 3.2 ± 0.2 | 4.0 ± 0.1 | 3.3 ± 0.2 |
| 5.37-C | 5.37 | Aβ1-39 | 1.8 ± 0.1 | 4.0 ± 0.8*** | 4.9 ± 0.4*** | 4.8 ± 0.6*** | 4.2 ± 0.9*** |
| 5.37-D | 5.37 | Aβ1-38 | 9.6 ± 1.9 | 12.4 ± 1.1 | 13.1 ± 1.3* | 13.4 ± 1.5* | 11.9 ± 2.5 |
| 5.37-E | 5.37 | Aβ1-37 | 5.7 ± 1.2 | 7.2 ± 1.2 | 8.5 ± 1.6 | 7.3 ± 1.2 | 6.8 ± 2.3 |
| 5.37-F | 5.37 | Aβ1-41 | 2.9 ± 1.6 | 2.7 ± 0.6 | 3.0 ± 0.3 | 3.7 ± 0.2 | 2.5 ± 0.7 |
| 6.37-A | 6.37 | Aβ2-40 | 1.7 ± 0,7 | 6.0 ± 1.1** | 4.2 ± 0.6 | 2.8 ± 1.1 | 6.4 ± 3.1*** |
| 6.37-B | 6.37 | Aβ2-42 | 0.6 ± 0.4 | 4.3 ± 1.2*** | 3.7 ± 0.8** | 2.5 ± 1.2* | 3.5 ± 1.5** |
| 6.37-F | 6.37 | n. i., Aβ2-40a |
8.4 ± 5.3 | 20.1 ± 5.9** | 11.4 ± 1.7 | 4.4 ± 1.5 | 10.0 ± 2.3 |
| 6.37-G | 6.37 | n. i. | 0.5 ± 0.4 | 4.7 ± 1.2*** | 3.8 ± 0.9*** | 2.6 ± 1.2* | 3.4 ± 1.6** |
| 5.45-A | 5.45 | n.i., Aβ2-40b |
1.3 ± 0.9 | 5.9 ± 1.3** | 6.7 ± 0.7*** | 5.3 ± 2.0** | 6.0 ± 2.9** |
Values are expressed as mean ± SD; *P < .05, **P < .01, ***P < .001 compared with CON (one-way ANOVA followed by Dunnett's multiple comparison test)
n. i. - not identified