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pSmad2 immunoreactivity is increased in the stroke penumbra and colocalizes with CD68 and GFAP. Representative images (10×) showing CD68 and GFAP colocalized with pSmad2 after stroke (image A is from a young female mouse at day 14 post stroke, image B is from a young female mouse after sham surgery). There is a qualitative increase in pSmad2 immunoflorescence in the infarcted region (outlined) with the brightest cells co-localizing with CD68 and GFAP.
