Table 1.
Characterization of reassortant viruses.
| virus | Genotyping on S7 amplicona |
Genotyping on S10 ampliconb |
BTV6 serotype specific PCRc |
BTV8 serotype specific PCRd |
|---|---|---|---|---|
| BTV8/net06 | 8 | 8 | - | + |
| BTV6/net08 | 6 | 6 | + | - |
| BTV6/net08/S78 | 8 | 6 | + | - |
| BTV6/net08/S108 | 6 | 8 | + | - |
a. S7 amplicons were digested with BglII and PstI and compared to that of the parental strains, see also figure 2, lanes 8 and 9. b. S10 amplicons were sequenced and compared to sequences of parental strains BTV8/net06 and BTV6/net08. Genetic serotyping by serotype-specific real-time PCR-assays was performed for serotypes 6 (c) and 8 (d). Presence or absence of a Cp-value was interpreted as + and -, respectively.