Figure 1.

ALCAM expression in tumor cells. A) Quantification of ALCAM mRNA by qRT-PCR. Data shown for each cell line is the mean of three analyses each in triplicate. B) Western blot analysis of ALCAM protein in the indicated cells lines. Whole cell lysates (20 μg) were probed with anti-ALCAM antibody, and the filter stripped and re-probed for α-tubulin. C) Immunocytochemical analysis for ALCAM expression and sub-cellular localization in breast cancer cell lines HCC1806 and HCC1500. Cells were stained for ALCAM (red) and nucleus (blue) using the DAPI reagent.