Figure 3.
ALCAM promoter activity in melanoma tumor cells. A) Schematic diagram of DNA constructs used for reporter gene assays depicting truncations of the ALCAM promoter cloned upstream of a promoter-less luciferase (Luc) gene. Histogram shows the relative luciferase activity for each construct in melanoma cell lines. B) Schematic diagram as described above, and activity of the p1200ALCAMLuc construct containing a wild-type (filled circle) or mutant (open circle) consensus binding sequence for NF-κB in the ALCAM promoter.