Figure 2.
Presence of cis and trans proline isomers in N-terminal sVpr. A1: Fingerprint region of the 2D 1H-1H TOCSY spectrum showing the assigned spin systems of residues close to proline residues in sVpr(1-20) and A2: Superimposed expanded HN-HN regions of the 2D 1H-1H NOESY spectra of sVpr1-20 in H2O:D2O (9:1, v/v) at pH 7 prior to (red signals) and after addition of 50 μl (0.1 mg) CypA (green signals). Notice the appearance of exchange peaks originating from enhanced prolyl cis/trans interconversion rate after addition of CypA. B1: Expanded Hα-Hβ region of the 2D 1H-1H TOCSY spectrum of sVpr21-40 in H2O:D2O (9:1, v/v) at pH 7 showing the signals of trans and cis Pro-35. B2: Superimposed expanded Hα-Hβ regions of the 2D 1H-1H NOESY spectra of sVpr21-40 in H2O:D2O (9:1, v/v) at pH 7 prior to (red signals) and after addition of 100 μl (0.2 mg) CypA (green signals). Notice the appearance of exchange peaks originating from enhanced prolyl cis/trans interconversion rate after addition of CypA.