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. 2010 Oct 4;10:31. doi: 10.1186/1472-6807-10-31

Figure 6.

Figure 6

Characterization of CypA binding to N-terminal peptides of sVpr. Synthetic Vpr1-20(A), Vpr21-40 (B), Vpr25-40 (C), Vpr1-40(D) and synthetic mutants carrying Pro to Asn exchanges at amino acid positions 5, 10 and 14(sVpr1-40P5,10,14N) (E) and at position 35 (sVpr1-40 P35N) (F) were tested for binding to immobilized recombinant CypA using a SPR biosensor system. A, B and C were injected at concentrations ranging from 0-400 μM over CM5 chip immobilized with 918 RU CypA, while D, E and F were injected at concentrations ranging from 0-200 μM. The curves A, B, C, D and E were best fit to a two state reaction model (Fig. 7). ka1, ka2, kd1, kd2 and KD were calculated for respective sensograms (Table 1).