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Optimized fit of SPR sensograms to binding models. The sensograms were fit to the binding models 1:1 (Langmuir) (A1-G1) and two-state reaction (conformational change) model (A2-G2). Black lines show the best curve fit in each instance. The curves originating from the interactions of sVpr21-40 (A), sVpr25-40 (B), sVpr1-40(C), sVpr1-40 P5,10,14N (D), sVpr30-40 (E), sVpr32-38 (F) and sVpr33-37(G) with CypA were considered to fit to two state reaction model (sensogram A2-G2) best.
