Figure 8.

Characterization of CypA binding region of the N-terminus of Vpr. Synthetic Vpr^30-40 (A), Vpr^32-38 (B), Vpr^33-37(C, D) were tested for binding to immobilized recombinant CypA using SPR biosensor system. Individual peptides were injected at concentration ranging from 0-400 μM over CM5 chip immobilized with 980 RU CypA. The curves were best fit to a two state model, and k_a1, k_a2, k_d1, k_d2 and K_D were calculated for the respective sensograms (Table 1.).