FIG. 3.

Synergy between PGN and poly(I:C) requires functional TLR2, MYD88, and TICAM1. Primary mouse peritoneal macrophages extracted from TLR2-deficient (Tlr2KO) and wild-type (C57BL/6J) control mice and from MYD88-deficient (Myd88KO), TICAM1-deficient (Ticam1KO), doubly deficient (DBL KO), and wild-type (B6129F2/J) controls were stimulated with either PGN (1 μg/ml), poly(I:C) (10 μg/ml), or both for 5 h. Duplex RT-PCR was performed for IL1B, TNF, and CCL5 (RANTES), normalized to the housekeeping gene GAPDH. Means ± SD are shown for triplicate wells. Average expression for wild-type macrophages treated with combined PGN plus poly(I:C) was set to 100. P values for the four treatment groups calculated by ANOVA are indicated separately for each genotype. Absent P values indicate unmeasurable values. Depicted is a representative example from among three repeat experiments.