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. 2010 Jul 21;83(5):807–817. doi: 10.1095/biolreprod.110.085613

FIG. 3.

FIG. 3.

The developmental regulation of Dusp6 mRNA expression and MAPK1/3 phosphorylation in the mouse epididymis. A) Real-time PCR quantification of Dusp6 mRNA expression level in the mouse epididymis from E14 to P7. Pro and dis represent proximal and distal epididymis, respectively. The average level of Dusp6 mRNA expression at P1 proximal epididymis (P1pro) was set as one. B) Quantification of Dusp6 mRNA expression level in the four regions of the epididymides from P14 to P21 (n = 3). **P < 0.01. The average level of Dusp6 mRNA expression in the IS at P14 was set as one. C) In situ hybridization of Dusp6 (top) and IHC staining of p-MAPK1/3 (bottom) of the proximal epididymis of P15, P17, P19, and 8-wk-old mice. Sense probe hybridization only revealed background level signal (data not shown).