Fig. 8.

Overexpression of Ets1 in cultured keratinocytes drives expression of Mmps and chemokines. (A) Retroviral constructs used to infect keratinocytes: MIGR1, control empty retrovirus; MIGR1-Ets1, retrovirus encoding mouse Ets1; MIGR1-R391D, retrovirus encoding the DNA-binding-defective mutant of Ets1. R391D represents a mutation in a key arginine residue of Ets1 required for DNA binding. This mutant version of Ets1 fails to bind DNA or regulate target genes. (B) RT-PCR analysis of Mmp and chemokine gene expression in retrovirally infected mouse keratinocytes. Ccl2, Ccl20 and Cxcl5 are the three chemokines tested; Mmp9 and Mmp13 are the two Mmps tested; Hprt is an internal control for RNA quantity and integrity.