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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Inflamm Bowel Dis. 2010 Nov;16(11):1859–1870. doi: 10.1002/ibd.21288

FIGURE 8.

FIGURE 8

Increased VEGF-A levels in Eng+/− mice under basal conditions and after DSS-induced colitis. A) Endoglin and VEGF levels were estimated in colonic protein samples fractionated under non-reducing conditions, expressed relative to β-actin and normalized to the level of untreated Eng+/+ mice. Endoglin was significantly reduced in Eng+/− samples and no changes were observed following DSS induction. VEGF levels were increased in Eng+/− vs. Eng+/+ mice, prior to DSS induction, with a further increase at day 19. B) Protein levels of VEGF-A estimated by ELISA were higher in Eng+/− than Eng+/+ mice under basal conditions and in the chronic phase of colitis (Days 19-26). C) mRNA levels estimated by real time PCR were significantly higher in Eng+/− than Eng+/+ mice prior to treatment and on day 19, following DSS treatment. #P < 0.05 vs. Eng+/+ mice at day 0; #P < 0.05 vs. DSS-treated Eng+/+ mice; †P < 0.05 vs. corresponding day 0 values. In Western blots, N = 4-6 mice for endoglin and N = 5-6 for VEGF; in ELISA, N = 9-11 for all groups; in real time PCR analysis, N = 4-5 samples for all groups, with a minimum of 4 replicates per sample.