Adhesion of hCB-ECs to SMCs depends upon α5β1 and αVβ3 integrins. A, Adhesion assays with hCB-ECs were performed as in Figure 1, except that hCB-ECs were pre-incubated with the indicated control or integrin-blocking IgG’s. Adherent ECs were scored and plotted as in Figure 1, for 4 independent experiments. Compared with control IgG: *, p < 0.01. B, ECs of the indicated type were subjected to cell surface immunofluorescence and flow cytometry to quantitate expression of the indicated integrins. Plotted are the mean ± S.E. from 4 independent experiments. Compared with PB-EC(CAD), PB-EC(healthy), and HAEC: *, p < 0.05.