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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 2;78(2):490–505. doi: 10.1111/j.1365-2958.2010.07346.x

Figure 5. Effects of Asp3 domain disruption upon the export of GspB736flag.

Figure 5

(A) Secondary structure analysis of Asp3. Locations of EZ-Tn5 insertions are shown above. (B) Western blot analysis of GspB736flag export from PS1244 derivative strains carrying in-frame insertions within Asp3. Culture media (M) and protoplasts (P) were collected from exponentially growing strains and prepared as described in the methods and materials. Proteins were separated by SDS-PAGE (3–8%) and following Western blot transfer, GspB736flag was probed with anti-flag antibody. (C) Western blot detection of Asp3 and the in-frame insertion mutants. Proteins from the protoplast fraction were separated by SDS-PAGE (4–12%) and probed with anti-Asp3 antibody.