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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 2;78(2):490–505. doi: 10.1111/j.1365-2958.2010.07346.x

Figure 7. Disruption of protein binding between Asp3 mutants and accessory Sec components.

Figure 7

Binding of Asp3 cysteine replacement mutants with (A) Asp2, (B) Asp3 and (C) SecA2 was assessed by yeast two-hybrid assays. Diploid yeast cells containing the indicated Asp3 mutant and interacting partner were grown on selective media containing galactose and assayed for β-galactosidase activity using ONPG as the substrate. The values shown are the geometric means ± SD of triplicate samples. * = P<0.01, for mutant versus WT.