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. Author manuscript; available in PMC: 2011 Oct 28.
Published in final edited form as: J Phys Chem B. 2010 Oct 28;114(42):13536–13544. doi: 10.1021/jp106406p

Figure 3.

Figure 3

FT-IR spectra of nitrile-modified RNase S and S-peptide. Top: normalized spectra of nitrile-modified S-peptide, free (blue) and bound to S-protein to form the RNase S complex (black) in 50% (vol/vol) glycerol in water, pH 7, 298 K. Bottom: Absorption spectra of the same set of RNase S complexes as above, taken at 80 K in black (left axis), and Stark spectra scaled to an applied field of 1 MV/cm in red (right axis), see Table 3.