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. Author manuscript; available in PMC: 2010 Oct 22.
Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5499–5505. doi: 10.4049/jimmunol.180.8.5499

FIGURE 2.

FIGURE 2

The specificity and affinity of mAbA9 for the EMPD region was determined with inhibition ELISA and SPRA. a, Competitive inhibition ELISA: mAbA9 was preincubated with increasing amounts of 6xEMPD peptide (from 10 to 100 molar 6xEMPD excess). No competition was achieved with soluble mouse IgE. Values are means ± SD of five inhibition experiments. b, Determination of the binding affinity: SPRA-analysis was performed, by injecting increasing amounts of mAbA9. Relative response units served as data points for the subsequent scatchard plot analysis. The slope of the deduced straight line equation was used to determine the binding affinity constant.