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To demonstrate the capacity of mAbA9 to bind surface expressed mIgE, FACS analysis was performed. FITC-labeled mAbA9 and rat anti-mouse soluble IgE recognize mIgE on cell line K46 mIgE+ in a comparable way. The isotype control as well as the untransfected cell line stained with FITC-labeled mAbA9 overlap with unstained cells.
