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. 2010 Oct 1;13(7):1023–1032. doi: 10.1089/ars.2010.3251

FIG. 1.

FIG. 1.

SIRT1 treated with concentrations of up to 0.5 mM GSNO displays attenuated stimulation by RSV (50 μM). SIRT1 was treated with DTT to ensure global cysteine reduction. Increasing concentrations of GSNO had no effect on basal SIRT1 (white bars) activity but did inhibit RSV stimulation (light gray bars). Reversal of SIRT1 oxidation by an additional reduction step reverted SIRT1 activity (dark gray bars) and RSV response (black bars) to control conditions indicating that GSNO induces a reversible OPTM that inhibits SIRT1 stimulation by RSV (A). GSNO inhibited RSV-stimulated SIRT1 activity with an IC50 of 69 μM (B). (n = 3; **p < 0.01 vs. GSNO-RSV; #p < 0.001 vs. GSNO-RSV).