Non-denaturing gel band shift
assay of the FRET samples. The concentration of the ss-1Acr strand
was 28 µM, those of A, B, A-5′BODIPY-TR
(A*) and B-5′BODIPY-TR (B*)
were 100 µM. Lane 1, ss-1Acr; lane 2,
A*B duplex; lane 3, AB* duplex; lane 4, ss-1Acr·B* duplex
with excess B* strand; lane 5, ss-1Acr·A*B
triplex; lane 6, ss-1Acr·AB* triplex; lane 7,
ss-1Acr·B duplex; lane 8, ss A*; lane 9, ss B*.
(A) Acridine fluorescence image of unstained gel
at 496 nm, detected with a bandpass SKF-9 filter and excitation
at 302 nm. (B) BODIPY-Texas Red fluorescence image
of the same gel as in (A). Emission at > 630 nm, excitation
at 366 nm.