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Uniformity of trafficking pattern to the MTOC in enucleated cells. Enucleated cells were prepared and infected as for Fig. 2. Following infection with carboxyfluorescein-Ad (green), enucleated cells were incubated for 1 h and then reinfected with Cy3-labeled Ad (red). The reinfected enucleated cells were then incubated for 0 or 60 min. Colocalization of the two different Ads is indicated in yellow in the overlay of images. Bar = 10 μm.
