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Point mutational analysis of a putative N-terminal NLS in 16E6 protein. 16E6*I and 16E6*II proteins were chosen for the assay because they have the same N-terminal 41 aa residues as the full-length 16E6 protein and could simplify the assay. The basic residues in the putative NLS were mutated to G or E residues, as indicated by arrows at the top. DIC, differential interference contrast; wt, wild type; mt, mutant. Scale bar, ∼8 μm.
