FIG. 6.

Mutational analysis of 16E6 NLS2 and NLS3 motifs in truncated 16E6 in COS-1 cells. The images were captured from cells expressing truncated 16E6, ΔN30+ΔC130, and ΔN56, with or without mutations in NLS2, NLS3, or both, at 24 h after transfection. The basic residues in the two NLS motifs were mutated to acidic residues, as indicated by arrows at the top. DIC, differential interference contrast; wt, wild type; mt, mutant. Scale bar, ∼8 μm.