FIG. 5.
Incorporation of Fc-G28.5 fusion protein into targeting vector complexes. Targeting complexes formed by association of the Fc-G28.5 ligand with either Ad5.DR-HI10-Cd, Ad5.DR-HI40-Cd, or Ad5.DR-LL-Cd were purified from unincorporated ligands on CsCl gradients, and aliquots of each preparation corresponding to 1.5 × 109 vp were analyzed by immunoblotting alongside samples of Ad vectors that were not incubated with Fc-G28.5. (A) Membrane probed with anti-fiber MAb; (B) result of the ligand detection done with Penta-His MAb. +, samples preincubated with ligand; −, samples containing Ad vectors only. Lane C shows a mixture of 1.5 × 109 vp of Ad5.DR with 12 ng of Fc-G28.5.