FIG. 1.

Analysis of effector function within ileac lymph nodes at early times postimmunization. Cells were obtained from the regional lymph nodes 3 to 6 days following intramuscular immunization with 10⁸ PFU of AdSIINFEKL-Luc. Upper panels, cells were stained directly with tetramer, and the number in the upper-right corner of each tetramer plot is the frequency of SIINFEKL-reactive CD8⁺ T cells (determined using the gate shown)/total CD8⁺ cells in the experimental group minus the frequency of SIINFEKL-reactive CD8⁺ T cells/total CD8⁺ cells in naïve controls; Middle panels, cells were immediately restimulated with SIINFEKL or KAVYNFATM peptide for intracellular cytokine staining, and the number in the upper-right corner is the frequency of IFN-γ-producing CD8⁺ T cells (determined using the gate shown)/total CD8⁺ following stimulation with SIINFEKL minus the frequency of IFN-γ-producing CD8⁺ T cells/total CD8⁺ cells following stimulation with KAVYNFATM; Lower panels, CFSE-labeled target cells were adoptively transferred into immunized recipient for in vivo CTL 4 h prior to harvesting tissues, and the number in the upper-right corner represents the percent specific lysis relative to naive controls. These histograms and density plots are representative of five mice, and the calculated values in all panels represent the means for the five mice.