TABLE 2.
Amino acid changes in triple-mutant Ad vector
| Vector | Sequence of penton base | Ad type of tail | Ad type of shaft | Amino acid sequence (nucleotide sequence) of knob domain
|
||
|---|---|---|---|---|---|---|
| FG loop | HI loop | C terminus | ||||
| Conventional Ad | MND-HAIRGDTFAT-RAE | 5 | 5 (22 β-repeats) | TEGTAYTNAV | DTTPSA (GAC ACA ACT CCA AGT GCA) | QE-Stop (CAA GAA TAA) |
| Triple-mutant Ad | MND-TS-RAE (RGD motif deleted) | 5 | 35 (6 β-repeats) | TEGNAV (aa 489 to 492 deleted) | DTTSNPSA (GAC ACA ACT TCG AAC CCA AGT GCA)a | QEID-Stop (CAA GAA ATC GAT TAA)b |
a
Underlining indicates an added Csp45I site. Two amino acids (S and N) were added to the sequence.
b
Underlining indicates an added ClaI site. Two amino acids (I and D) were added to the sequence.