FIG. 4.
Estimation of EBV genome copy number in MDA-MB468 cells infected in vitro. Real-time quantitative PCR was performed in duplicate with primers specific for the EBV BamHI-W fragment. (A) Amplification plots of the fluorescence intensity against PCR cycle number are shown to compare the EBV genome load in MDA-MB468 and virus-negative Akata (−) B cells 48 h after infection with cell-free BX1 virus. (B) EBV genome load in uninfected and infected MDA-MB468 cells versus EBV-positive Raji B cells. x axis, PCR cycle number. y axis, fluorescence intensity.