FIG. 5.

Southern blot analysis of 6TG-resistant HT-1080 clones. Genomic DNAs from 15 6TG-resistant clones (5 each from cells transduced with MVM-HPe3TNA-f, MVM-HPe3TNA-r, and the mixture of vectors) and untransduced, parental HT-1080 cells were digested with HindIII (A and B) or EcoRI (C and D) and probed for HPRT (A and C) or neo (B and D) sequences. The positions of size standards are shown to the left of each panel, and the predicted fragment sizes of wild-type and targeted alleles are indicated. The locations of restriction sites and the probe fragments are shown in Fig. 1.