TABLE 1.
Vector | G418 resistancea
|
6TG resistance
|
||||||
---|---|---|---|---|---|---|---|---|
No. of integration sites/cell
|
4.1-kb BglII fragment | No. with concatemer | No. of clones analyzed | No. of expt. | No. (%) of clones
|
|||
Single | Multiple | Targeted | Targeted + random | |||||
Forward | 7 | 3 | 4 | 1 | 20 | 3 | 17 (85) | 0 (0) |
Reverse | 4 | 6 | 3 | 1 | 12 | 3 | 10 (83) | 7 (70) |
Mixture | 5 | 5 | 2 | 1 | 10 | 1 | 10 (100) | 2 (20) |
10 clones analyzed for each vector.