FIG. 1.

VLP interaction with HSPGs. (A) VLPs were bound to heparin-BSA-coated microtiter plates in the presence of soluble heparins of different molecular masses (H-4784 [16 kDa], H-5284 [6 kDa], and H-3400 [3 kDa]) or de-N-sulfated (D-4776), de-O-sulfated/acetylated (A-6039), or acetylated heparin (A-8036). Bound VLPs were determined. Binding in the absence of glucosaminoglycan was set as 100%. (B) VLP binding to COS7 cells in the presence of heparin derivatives. Bound VLPs were monitored by Western blot with L1-specific antibody 33L1-7.