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. 2003 Dec;77(24):12961–12967. doi: 10.1128/JVI.77.24.12961-12967.2003

FIG. 4.

FIG. 4.

Interference of monoclonal antibodies with VLP binding to heparin. (A) Titration of two HPV33 VLP-specific monoclonal antibodies (H33.J3 and H33.B6) with HPV33 L1L2 VLPs bound to heparin-BSA-coated microtiter plates. (B) VLPs were incubated with monoclonal antibodies H33.B6 and H33.J3 prior to addition to heparin-BSA-coated plates. The amounts of bound VLPs were determined by using horseradish peroxidase-labeled antibodies. Antibody H33.B6 but not H33.J3 interferes with binding to glucosaminoglycans.