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. 2001 Feb 15;29(4):1005–1011. doi: 10.1093/nar/29.4.1005

Figure 2.

Figure 2

SDS–PAGE (12.5%) demonstrating solubility and purity of TniA expressed in E.coli BL21(DE3). The protein bands were stained with Coomassie Brilliant Blue R. Lane 1, molecular mass standard (molecular weights given in kDa); lane 2, insoluble proteins in a crude extract of BL21(DE3); lane 3, soluble proteins in a crude extract of BL21(DE3); lane 4, insoluble proteins in an extract from BL21(DE3) expressing TniA from pLKO420; lane 5, soluble proteins in an extract from BL21(DE3) expressing TniA from pLKO420; lane 6, pooled and concentrated TniA peak fractions purified by ion exchange chromatography; lane 7, TniA further purified by gel filtration.