Figure 1.
Translesion DNA synthesis opposite a template 8-oxoguanine (8-oxoG) by human Pol ι. (A) The DNA template for translesion synthesis. The 17mer primer was labeled with 32P at its 5′-end as indicated by an asterisk and annealed right before the lesion. (B) DNA polymerase assays were performed with increasing amounts of purified human Pol ι using DNA templates without (lanes 1–5) or with (lanes 6–10) a site-specific 8-oxoguanine. DNA size markers in nt are indicated in the middle. (C) Quantitation of the results in (B).