Figure 2.

Specificity of nucleotide incorporation by human Pol ι opposite the template 8-oxoguanine. DNA polymerase assays were performed with purified human Pol ι (19 ng, 238 fmol) using 50 fmol of DNA templates without (lanes 1–5) or with (lanes 6–10) a site-specific 8-oxoguanine. The 8-oxoguanine DNA template containing a ³²P-labeled 17mer primer annealed right before the lesion is shown in Figure 1A. Polymerase reactions were carried out in the presence of each deoxyribonucleoside triphosphate dATP (A), dCTP (C), dTTP (T) and dGTP (G) individually or all four dNTPs (N₄). Quantitation of extended primers is shown at the bottom of the gel. DNA size markers in nt are indicated on the left.