Figure 4.

Efficient nucleotide insertion by human Pol ι opposite a template AP site. A ³²P-labeled 17mer primer was annealed right before an A of the undamaged template FY3 or annealed right before the AP site (designated by X) of the template AP as shown. Polymerase assays were performed with increasing amounts of human Pol ι using 50 fmol undamaged template (FY3, lanes 1–3) and the AP site-containing template (template AP, lanes 4–6). Quantitation of extended primers is shown at the bottom of the gel. DNA size markers in nt are indicated on the right.