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Nucleotide incorporation opposite a template AAF-guanine by human Pol ι. A 32P-labeled 17mer primer was annealed right before the template AAF-G as shown. DNA polymerase assays were then performed with 37 ng (463 fmol) purified human Pol ι using dATP (A), dCTP (C), dTTP (T) and dGTP (G) individually or all four dNTPs (N4). DNA size markers in nt are indicated on the right.
