FIG. 10.

Transient infection of wt (KH-SV) or α6 integrin-deficient (BOUA-SV) keratinocytes with native HPV11 virions (RT-PCR infectivity assay). Cells were infected with native HPV11 virions or left untreated and incubated for 3 days. Total RNA was isolated and E1-E4 transcripts were amplified by RT-PCR. Amplicons were separated by gel electrophoresis and visualized by ethidium bromide staining. Neutralization with an anti-HPV11 MAb (H11.B2) and amplification of β-actin served as controls, as indicated.