FIG. 2.

Papillomavirus infectivity assays (RT-PCR) of K562 transfectants expressing an empty eukaryotic vector (K562 vector), syndecan-1 (K562 SD-1), syndecan-4 (K562 SD-4), or glypican-1 (K562 GP-1) or of HaCaT cells. Cells were infected with the indicated amounts of HPV11 (A and B) or 0.2 μl (5 focus-forming units) of BPV (C) virions for 1 h, incubated for 3 days, and analyzed for E1-E4 spliced viral RNA expression by RT-PCR. Amplicons were separated by gel electrophoresis and visualized by ethidium bromide staining. Neutralization of virions with anti-HPV11 MAb (H11.B2), infection of HaCaT keratinocytes, PCR in the absence of cDNA (H₂O), and amplification of β-actin mRNA served as appropriate controls as indicated.