FIG. 5.

Inhibition of HPV16 VLP binding to human keratinocytes (KH-SV) by treatment with heparitinase, but not by PLC. Keratinocytes (KH-SV) were incubated with increasing concentrations of heparitinase or PI-PLC (13 U/ml) or left untreated, as indicated. Following a wash step with PBS, cells were incubated with (or without) VLP for 1 h and washed extensively, and cell-VLP complexes were analyzed by SDS-PAGE and Western blotting using MAb Camvir-1. The band at ∼45 kDa (indicated by a star) represents a cellular protein cross-reacting with MAb Camvir-1.