TABLE 3.

Toto:ts138ABs recombinants resemble ts138 and are not ts for 26S mRNA synthesis^a

Virus	Molar ratio of 49S RNA:26S mRNA
	At 30°C	At 40°C when shifted up at:
		1 h p.i.	4 h p.i.	8 h p.i.
ts138	0.12	0.19	0.16	0.19
Toto:ts138ABs
1	0.19	0.28	0.21	0.27
4	0.16	0.29	0.21	0.26

^aCEF cells were infected with each virus at an MOI of 100 PFU per cell. At 4 and at 9 h p.i., one set of cultures was labeled for 1 h at 30°C with [³H]uridine (50 μCi/ml). At the times indicated, cultures were shifted to 40°C and incubated for 1 h before being labeled for 1 h at 40°C. At the end of the labeling period, the monolayers were harvested and the lysates were electrophoresed on agarose gels as described in Materials and Methods. The molar ratio of the two RNAs was calculated from the incorporation present in each RNA band excised from the dried gel after correcting for their respective molecular weights.