FIG. 1.

Viral protein synthesis during IFN treatment. 293T cells were pretreated with various amounts of IFN-α2a (0, 5, 10 50, 100, and 500 U/ml) for 16 h and were then transfected with proviral DNA, pACH, in the presence of IFN-α2a for 72 h. The same amount of protein from cell lysates and supernatants was loaded in SDS-10% polyacrylamide gel electrophoresis. Anti-HTLV-1 human MAb against p19^Gag and polyclonal antibody against cellular actin were used to react with blots. IFN reduced virion p19^Gag in cell supernatants in a dose-dependent manner (A) and had no effect on the viral structural protein Gag, and cellular actin in cell lysates (B). IFN significantly reduced p19^Gag production in supernatants, based on quantitation of virion production with p19 ELISA. Representative data from four independent experiments are indicated in panel C. The bar labeled “con” indicated mock-transfected cells.