TABLE 1.
Titer and mean recombination frequency after coinfection of virus A and virus G at an MOI of 5 for each virus
Time (h) postcoinfection | Titer (PFU/ml) | Total no. of plaques and phenotype
|
Mean recombination frequencya (%) ± SD | |||
---|---|---|---|---|---|---|
polh−
|
polh+
|
|||||
lacZ− | lacZ+ | lacZ− | lacZ+ | |||
2 | 5.6 × 104 | 231 | 0 | 0 | 262 | 0 ± 0 |
12 | 1.2 × 105 | 182 | 0 | 0 | 268 | 0 ± 0 |
15 | 2.5 × 105 | 171 | 26 | 21 | 210 | 11.0 ± 0.5 |
18 | 2.7 × 106 | 250 | 88 | 110 | 258 | 28.3 ± 2.6 |
24 | 2.1 × 107 | 207 | 93 | 99 | 292 | 28.5 ± 3.3 |
48 | 1.6 × 108 | 190 | 45 | 71 | 163 | 24.7 ± 1.1 |
That is, [(polh− lacZ+ + polh+ lacZ−) ÷ (polh− lacZ− + polh− lacZ+ + polh+ lacZ− + polh+ lacZ+)] × 100%.