TABLE 3.
Titer and mean recombination frequency following coinfection of virus A and virus G at an MOI of 50, 1, or 0.1 for each virus
| Time (h) postcoinfection | MOI | Titer (PFU/ml) | Total no. of plaques and phenotype
|
Mean recombination frequencya (%) ± SD | |||
|---|---|---|---|---|---|---|---|
|
polh−
|
polh+
|
||||||
| lacZ− | lacZ+ | lacZ− | lacZ+ | ||||
| 18 | 50:50 | 4.1 × 106 | 164 | 68 | 85 | 167 | 31.7 ± 0.9 |
| 1:1 | 2.0 × 105 | 129 | 27 | 41 | 91 | 25.4 ± 5.0 | |
| 0.1:0.1 | 4.8 × 103 | 154 | 9 | 13 | 126 | 7.3 ± 0.8 | |
| 24 | 50:50 | 3.3 × 107 | 66 | 36 | 50 | 98 | 33.7 ± 4.4 |
| 1:1 | 7.1 × 106 | 95 | 24 | 29 | 80 | 23.3 ± 2.7 | |
| 0.1:0.1 | 1.9 × 105 | 147 | 12 | 11 | 99 | 8.6 ± 0.6 | |
| 36 | 50:50 | 1.6 × 108 | 73 | 33 | 39 | 82 | 32.1 ± 3.2 |
| 1:1 | 1.0 × 108 | 151 | 29 | 25 | 107 | 17.6 ± 4.6 | |
| 0.1:0.1 | 7.8 × 106 | 149 | 6 | 9 | 103 | 5.6 ± 0.3 | |
| 48 | 50:50 | 2.0 × 108 | 80 | 50 | 34 | 81 | 34.5 ± 1.1 |
| 1:1 | 2.0 × 108 | 123 | 22 | 29 | 104 | 18.3 ± 0.5 | |
| 0.1:0.1 | 6.2 × 107 | 115 | 19 | 24 | 120 | 16.3 ± 2.8 | |
That is, [(polh− lacZ+ + polh+ lacZ−) ÷ (polh− lacZ− + polh− lacZ+ + polh+ lacZ− + polh+ lacZ+)] × 100%.