TABLE 4.
Time (h) postcoinfection | MOI | Titer (PFU/ml) | Total no. of plaques and phenotype
|
Mean recombination frequencya (%) ± SD | |||
---|---|---|---|---|---|---|---|
polh−
|
polh+
|
||||||
lacZ− | lacZ+ | lacZ− | lacZ+ | ||||
18 | 50:50 | 7.9 × 106 | 238 | 4 | 7 | 213 | 2.4 ± 0.2 |
0.1:0.1 | 4.6 × 103 | 170 | 0 | 1 | 116 | 0.3 ± 0.4 | |
24 | 50:50 | 3.3 × 107 | 111 | 8 | 5 | 123 | 4.6 ± 3.3 |
0.1:0.1 | 2.8 × 105 | 140 | 0 | 0 | 96 | 0.0 ± 0.0 | |
36 | 50:50 | 1.6 × 108 | 120 | 6 | 8 | 101 | 5.3 ± 1.7 |
0.1:0.1 | 1.0 × 107 | 147 | 1 | 1 | 95 | 0.9 ± 0.3 | |
48 | 50:50 | 2.5 × 108 | 133 | 6 | 5 | 111 | 4.4 ± 1.2 |
0.1:0.1 | 6.2 × 107 | 138 | 4 | 4 | 85 | 4.0 ± 2.8 |
That is, [(polh− lacZ+ + polh+ lacZ−) ÷ (polh− lacZ− + polh− lacZ+ + polh+ lacZ− + polh+ lacZ+)] × 100%.