TABLE 6.
Titer and recombination frequency after oral coinfection of third-instar H. virescens with 3,000 polyhedra from AcMNPV and virus G
Time (h) postcoinfection | Titer (PFU/ml of hemolymph) | Total no. of plaques (phenotype)
|
Recombination frequencya (%) ± SD | |
---|---|---|---|---|
Wild-type AcMNPV (polh+lacZ−) | Recombinant (polh+lacZ+) | |||
72 | 2.8 × 108 | 170,000 | 51 | 0.02 ± 0.02 |
120 | 8.2 × 108 | 139,000 | 55 | 0.03 ± 0.02 |
That is, [(polh+ lacZ+) ÷ (polh+ lacZ− + polh+ lacZ+)] × 100%.