TABLE 2.

Replication of wild-type virus and ICP0 Δ621-625 in actively dividing and resting pHF fibroblasts^a

pHF cells	Yield (PFU, 105)
	HSV-1(F)	R6701
Actively dividing	1,500	1,300
Serum starved	70	5.0
Contact inhibited	800	29

^aDiploid HFF fibroblasts grown in 25-cm² flasks were infected with HSV-1 while actively divding before reaching confluence or either allowed to become fully confluent (contact inhibited) and maintained in the same medium for 7 days or serum starved in medium containing 0.25% fetal calf serum for 5 days prior to infection. Cells were exposed to 0.05 PFU of HSV-1 per cell in the same medium in which they were maintained prior to infection and harvested at 20 h after infection.